Improvement effect of active ingredients of Anemarrhena asphodeloides on sepsis-induced myocardial injury model mice / 中国药房

OBJECTIVE To provide reference for clarifying improvement effects of Anemarrhena asphodeloides on sepsis- induced myocardial injury and potential material basis. METHODS Water extract of A. asphodeloides was extracted by thermal reflux method. Total xanthone and total saponins in A. asphodeloides were separated by macroporous adsorption resin. The mice model of sepsis-induced myocardial injury was established by intraperitoneal injection of lipopolysaccharide. The effects of the location of three extraction fractions and the monomers of A. asphodeloides as mangiferin， timosaponin AⅢ and timosaponin BⅡ on the survival rate of the model mice were explored. HE staining was used to observe the effects of mangiferin， timosaponin AⅢ and timosaponin BⅡ on myocardial morphology in model mice. The effects of mangiferin on mRNA expressions of inflammatory cytokines [interleukin-6 （IL-6）， IL-1β and tumor necrosis factor-α （TNF-α）] and the level of reactive oxygen species （ROS） in myocardial tissue of model mice were detected. RESULTS Compared with the model group， the survival rate of mice in the intervention group of total xanthone， total saponins and water extract was increased to different extents， especially total xanthone fraction. Mangiferin， timosaponin AⅢ and timosaponin BⅡcould improve the degree of myocardial cell swelling and muscle bundle arrangement disorder in model mice， especially mangiferin. Compared with model group， mRNA expressions of IL-6， IL- 1β and TNF- α， ROS level in myocardium of mice after mangiferin intervention were decreased to different extents. CONCLUSIONS The different extraction fractions of A. asphodeloides can improve survival rate of mice with sepsis-induced myocardial injury， especially total xanthone fraction. Mangiferin is the best among the three monomers of A. asphodeloides to improve sepsis-induced myocardial injury， which may play a role in anti-sepsis myocardial injury by anti-inflammation and antioxidantion.

Research on autophagy induced by two xanthone compounds in HepG2 cells / 中国中药杂志

To investigate the inhibitory effects of two xanthone compounds, 1-hydroxy-2,3,4,8-4 methoxy xanthone(here in after referred to as Fr15) and 1-hydroxy-2,3,4,6-4 methoxy xanthone(here in after referred to as Fr17), on the proliferation of hepatocellular carcinoma cells HepG2, and to further investigate their mechanism in combination with transcriptomics. Cell counting was used to detect the effects of two kinds of xanthone compounds Fr15 and Fr17(0, 0.03, 0.15, 0.3 mmoL·L~(-1)) on the proliferation of HepG2 cells; the effects of the two compounds Fr15 and Fr17 on HepG2 cell cycle were detected by flow cytometry; the changes of autophagosomes count in cells were observed under fluorescence microscope; the expression of autophagy marker proteins autophagy marker proteins SQSTM 1(p62) and microtubule associated protein 1 light chain 3 Ⅰ/Ⅱ(LC3 Ⅰ/Ⅱ) in the cells was detected by Western blot; the differentially expressed genes between the control group and the experimental group were analyzed by RNA-seq transcriptome sequencing; qRT-PCR was used to verify the differentially expressed genes in sequencing. The results showed that compounds Fr15 and Fr17 inhibited the proliferation of HepG2 cells with the increase of drug concentration and time. Flow cytometry showed that compounds Fr15 and Fr17 had little effect on HepG2 cell cycle. Fluorescence microscopy results showed that the number of autophagosomes in cells increased with the increase of drug concentration. Western blot showed that the expression of p62 protein was decreased and the expression of LC3-Ⅱ protein was significantly increased after drug addition. The results of RNA sequencing showed that 26 102 and 52 351 differentially expressed genes were obtained in Fr15 and Fr17 respectively. Analysis of KEGG showed that drug treatment had a great effect on autophagy pathway. qRT-PCR verified that 6 up-regulated genes were related to autophagy, and their trend was consis-tent with sequencing results, where all 6 genes showed an up-regulated trend. Two xanthone compounds Fr15 and Fr17 may inhibit proliferation of HepG2 cells by inducing autophagy.

Chemical constituents and antibacterial activities of Aspidistra typica / 中国天然药物

In the present investigation, we carried a phytochemical study of an ethanol-soluble extract from the root barks of Aspidistra typica Baill., a traditional food and herb medicine, leading to the isolation of four different kinds of compounds, including one benzene substituted coumarin, two organic phosphonic compounds, and one xanthone. The novel benzene substituted coumarin typicacoumarin A (1) was a new type of coumarin, and the two new organic phosphonic compounds, typicalphosphine A (2) and typicalphosphine B (3) were isolated for the first time, and their structures were elucidated by spectral techniques, viz.1D, 2D NMR spectra and HR-ESI-MS. The xanthone compound typicaxanthone A (4) was discovered from nature for the first time. The two new organic phosphonic chlorides (2, 3) showed stronger antibacterial activities, which were comparable to berberine hydrochloride. And typicaxanthone A (4) showed much stronger antibacterial activity against Escherichia coli ATCC-25922 bacterial strain, while typicacoumarin A (1) showed moderate antibacterial activities, weaker than berberine hydrochloride.

Chemical constituents and antibacterial activities of Aspidistra typica / 中国天然药物

In the present investigation, we carried a phytochemical study of an ethanol-soluble extract from the root barks of Aspidistra typica Baill., a traditional food and herb medicine, leading to the isolation of four different kinds of compounds, including one benzene substituted coumarin, two organic phosphonic compounds, and one xanthone. The novel benzene substituted coumarin typicacoumarin A (1) was a new type of coumarin, and the two new organic phosphonic compounds, typicalphosphine A (2) and typicalphosphine B (3) were isolated for the first time, and their structures were elucidated by spectral techniques, viz.1D, 2D NMR spectra and HR-ESI-MS. The xanthone compound typicaxanthone A (4) was discovered from nature for the first time. The two new organic phosphonic chlorides (2, 3) showed stronger antibacterial activities, which were comparable to berberine hydrochloride. And typicaxanthone A (4) showed much stronger antibacterial activity against Escherichia coli ATCC-25922 bacterial strain, while typicacoumarin A (1) showed moderate antibacterial activities, weaker than berberine hydrochloride.

Analysis on chemical constituents from Swertia mileensis by UPLC-ESI-Q-TOF-MS / 中草药

Objective: To analyze and identify the chemical constituents from Swertia mileensis by UPLC-ESI-Q-TOF-MS. Methods: The analysis was performed on an Acquity HSS T3 reverse phase column (100 mm × 2.1 mm, 1.8 μm). The mobile phase consisted of 0.1% formic acid acetonitrile and 0.1% formic acid, and was used for gradient elution, with the flow rate of 0.4 mL/min. Mass spectrometry was applied for the qualitative analysis under positive and negative ion modes and ESI ion source. Data was analyzed by Masslynx 4.1 software, SciFinder database, literatures, and standards. Results: Twenty-eight compounds, including 7 iridoids, 14 xanthones, 3 flavonoids, 2 triterpenes, and 2 phenols were identified from S. mileensis. Among them, vogeloside, 8-O-β-D- glueopyranosyl-(l→6)-β-D-glueopyranosyl-l,7-dihydroxy-3-ethoxyxanthone, 3-O-demethylswertipunicoside, and sweriyunnanlactone A were reported from this species for the first time. Conclusion: Using UPLC-ESI-Q-TOF-MS method the main chemical constituents from S. mileensis can be rapidly and accurately identified, which provides a new strategy for its quality control as well as a reference for clarifying the material basis of its efficacy.

Chemical constituents of xanthone in Swertia kingii / 中草药

Objective: To study the chemical constituents of the roots of Swertia kingii. Methods: The chemical constituents were isolated and purified by silica gel and Sephadex LH-20 chromatographies. Their structures were identified on the basis of physicochemical properties and spectrometric data. Results: Ten compounds obtained from this plant were identified as demethylbellidifolin (1), β-sitosterol (2), 1,3,7-trihydro-xanthone (3), swertianolin (4), 1,3,7-trihydrox-8-methoxy-xanthone (5), 1,5,8- trihydroxy-3-methoxy-xanthone (6), 1,3,7,8-tetrahydroxyxanthone-1-O-β-D-glucopyranoside (7), 1,3,7,8-tetrahydroxy-xanthone (8), 1-hydroxy-3,7,8-trimethoxy-xanthone (9), and 1,2,6,8-tetrahydroxy-xanthone (10). Conclusion: Except for compound 2, the other compounds are obtained from this plant for the first time.

Simultaneous determination of eight components in root of swertia kingii by hplc / 中草药

Objective To establish an HPLC method for simultaneously determining eight components, such as sweroside, gentiopicroside, isoorientin, 1,3,7,8-tetrahydroxy-xanthone-1-O-β-D-glucopyranosyl, swertianolin, 1,3,7,8-tetrahydroxy-xanthone, demethylbellidifolin, and 1,5,8-trihydrox-3-methoxyxanthone in the root of Swertia kingii. Methods Chromatographic analysis was achieved on an Agilent Zorbax ODS column (250 mm × 4.6 mm, 5 μm) by gradient elution of acetonitrile-0.5% phosphoric acid in water at 30 ℃. The flow rate was 1.0 mL/min and the detection wavelength was 254 nm. Results The calibration curves of all the eight constituents showed good linearity in a relatively wide concentration range. The linear ranges of swertiamarin, gentiopicroside, isoorientin, 1,3,7,8-tetrahydroxy-xanthone-1-O-β-D-glucopyranosyl, swertianolin, 1,3,7,8-tetrahydroxy-xanthone, demethylbellidifolin, and 1,5,8-trihydrox-3-methoxyxanthone were 3.84-96.00 μg/mL (r = 0.999 6), 3.36-84.00 μg/mL (r = 0.999 2), 5.92-148.00 μg/mL (r = 0.999 8), 4.81-118.00 μg/mL (r = 0.999 2), 4.32-108.00 μg/mL (r = 0.999 3), 4.16-104.00 μg/mL (r = 0.999 2), 5.12-128.00 μg/mL (r = 0.999 4), 4.80-120.00 μg/mL (r = 0.999 6), respectively. The average recoveries were 99.59% (RSD 0.99%), 98.95% (RSD 3.37%), 98.61% (RSD 1.87%), 99.63% (RSD 1.93%), 99.31% (RSD 1.21%), 99.50% (RSD 1.62%), 99.80% (RSD 0.54%), and 98.50% (RSD 1.87%). Conclusion This method is simple, efficient, accurate, and reproducible, and can be used for the determination of eight constituents in root of Swertia kingii. This will promote the comprehensive usage of this plant.

Design, synthesis and biological activities of novel 3,6-diamide xanthones / 药学学报

A series of novel xanthones with terminal amine substituents at xanthone's C3 and C6 positions were designed and synthesized as potential ligands for telomeric G-quadruplex DNA. All the compounds in this series were bound to telomeric G-quadruplex in a "thread intercalation" manner that illustrated both in molecular docking and spectrometric studies. Among them, 10c and 10d showed better binding abilities and specific affinity toward G-quadruplex DNA HTG21 over ctDNA in the fluorescence assay. The antiproliferative activities of four screened compounds were examined in three cancer cells by MTT in vitro, and their inhibitory effects were observed at low micromolar ranges. In addition, the PCR stop assay demonstrated that 10c and 10d effectively inhibited the amplification ability of telomerase.

Chemical constituents from petroleum ether fraction of Swertia chirayita and their activities in vitro / 中国中药杂志

The present work is to study the chemical constituents from petroleum ether fraction of Tibetan medicine Swertia chirayita by column chromatography and recrystallization. The structures were identified by physical and chemical properties and spectral data as swerchirin (1), decussatin (2), 1,8-dihydroxy-3,5,7-trimethoxyxanthone (3), 1-hydroxy-3,5,7,8-tetramethoxyxanthone (4), bellidifolin (5), 1-hydroxy-3, 7-dimethoxyxanthone (6), methylswertianin (7), 1-hydroxy-3,5-dimethoxyxanthone (8), erythrodiol (9), oleanolic acid (10), gnetiolactone (11), scopoletin (12), sinapaldehyde (13), syringaldehyde (14), and β-sitosterol (15). Compounds 3, 4, 9, 11-14 were isolated from S. chirayita for the first time. Compounds 9 and 12 were firstly isolated from the genus Swertia. The cytotoxic activities of compounds 1, 2, 5, 7 and 8 against human pancreatic cancer cell lines SW1990 and BxPC-3,and the protective effects of these compounds against hydrogen peroxide (H2O2)-induced oxidative stress in human endothelium-derived EA.hy926 were investigated in vitro. The results showed no obvious effect at the high concentration of 50 μmol•L⁻¹.

Simultaneous Determination of Five Xanthones in Swertia chirayita by HPLC-ESI-MS / 中国药房

OBJECTIVE:To establish a method for the simultaneous determination of 5 xanthones in Swertia chirayita. METH-ODS:HPLC-EST-MS was conducted. The chromatographic conditions:the column was Waters Symmetry-C18 with mobile phase of water (5 mmol/L ammonium acetate- 0.1% formic acid)-methanol (gradient elution) at a flow rate of 0.8 ml/min,the detection warelength was 254 nm,column temperature was 30 ℃,and the injection volume was 10 μl;MS conditions:it was detected by positive ion,ion source ejection voltage was 4.5 kV,capillary temperature was 200 ℃,capillary voltage was 25 V,sheath gas flow rate was 35 arb,auxiliary gas flow rate was 20 arb,collision gas was helium,and detection method was multi-stage full scan mass spectrometry. RESULTS:The linear ranges for 1-hydroxy-3,5-dimethoxyxanthone,1-hydroxy-3,4,5-trimethoxyxanthone,1, 8-dihydroxy-3,dimethoxyxanthone,1-hydroxy-2,3,4,5-tetramethoxyxanthone and 1-hydroxy-2,3,4,7-tetramethoxyxanthone were all 0.5-15.00 μg/ml(r>0.999 0);RSDs of precision,stability and reproducibility tests were lower than 2%;recoveries were 100.03%-103.59%(RSD=0.99%,n=9)for 1-hydroxy-3,5-dimethoxyxanthone,97.41%-100.89%(RSD=1.15%,n=9)for 1-hy-droxy-3, 4, 5-trimethoxyxanthone, 99.13% -101.68%(RSD=1.40% , n=9) for 1, 8-dihydroxy-3, 5-dimethoxyxanthone, 100.21%-103.51%(RSD=1.33%,n=9)for 1-hydroxy-2,3,4,5-tetramethoxyxanthone,100.56%-103.92%(RSD=1.37%,n=9) for 1-hydroxy-2,3,4,7-tetramethoxyxanthone. CONCLUSIONS:The method is simple,stable and reproducible,and can be used for the simultaneous determination of 5 xanthones in S. chirayita.

Glycosides constituents of whole plant of Swertia binchuanensis / 中国药学杂志

OBJECTIVE: To study the chemical constituents of Swertia binchuanensis. METHODS: The constituents were isolated and purified by column chromatography of silica gel. Their structures were identified on the basis of spectral analysis and chemical properties. RESULTS: Five compounds are isolated and identified as 7-O-[α-L-rhamnopyranosyl-(1→2)-β-D-xylopyranosyl]-1, 8-dihydroxy-3-methoxyxanthone(1), 3-O-β-D-glucopyranosyl-1,8-dihydroxyl-5-methoxyxanthone(2), 7-O-β-D-glucopyranosyl-1,8-di-hydroxyl-3-methoxyxanthone(3), amarogentin(4), and amaroswerin(5). CONCLUSION: All of the compounds were isolated from S. binchuanensis for the first time.

Improvement of Content Determination for Polygala XanthoneⅢin Polygalae Radix / 中国药房

OBJECTIVE:To improve the content determination for polygala xanthone Ⅲ in Polygalae Radix contained in Chi-nese Pharmacopoeia (2010 edition). METHODS:HPLC was performed on the column of Hypersil BDS C18 with mobile phase of acetonitrile-0.05% phosphoric acid(gradient elution)at a flow rate of 1.0 ml/min;detection wavelength was 320 nm,column tem-perature was 25℃,and the injection volume was 10μl. RESULTS:The linear range of polygala xanthoneⅢwas 0.029-0.928 μg/ml (r=0.999 1);RSDs of precision,stability and reproducibility tests were lower than 2.0%;recovery was 94.66%-100.90%(RSD=2.46%,n=6). CONCLUSIONS:The method is simple,stable and reproducible. Although the determination time is prolonged,it has improved the accuracy and it is more suitable for the content determination of polygala xanthoneⅢin Polygalae Radix.

Antioxidant and tyrosinase inhibition activities of α- mangostin and Garcinia mangostana Linn. pericarp extracts.

The methanol, ethyl acetate and petroleum ether crude extracts of mangosteen pericarp and α- mangostin were evaluated for the antioxidant capacity and tyrosinase inhibition properties. The ferric reducing antioxidant power (FRAP) assay was used to investigate their antioxidant capacity. Tyrosinase inhibition effect was evaluated using mushroom tyrosinase inhibition assay. Methanol extract has higher antioxidant reducing capacity (m= 1.621), compared to the rest of the extracts. Meanwhile, ethyl acetate extract and α- mangostin showed potent tyrosinase inhibition activities as compared to Kojic acid, a well- known tyrosinase inhibitor. It is observed that tyrosinase inhibition effect is antioxidant independent as ethyl acetate extract possessed low antioxidant capacity. This study suggests direct tyrosinase inhibition by ethyl acetate extract of Garcinia mangostana.

Banfoxanthone, a new prenylated xanthone from the stem bark of Garcinia ovalifolia (Guttiferaceae).

A detailed chemical investigation of the secondary metabolites from the extract of the stem bark of Garcinia ovalifolia led to the isolation of one new prenylated xanthone named banfoxanthone and five known compounds, namely friedeline, canophyllal, ovalifoloneA, 3-methoxycheffouxanthone, endodesmiadol. Prenylated xanthones isolated from Garcinia ovalifolia and others species of genus Garcinia appear to be useful as chemotaxonomic markers in Garcinia.

Cytotoxic compounds from the leaves of Garcinia cowa Roxb.

The aims of this study was to isolate compounds from the leaves of methanol extract of Garcinia cowa and to evaluated their cytotoxic activity against breast (MCF-7) and lung (H-460) cell lines. The dichloromethane fraction was separated by successive silica gel column chromatography to give three compounds. Based on spectroscopic comparison with those of the literature these compounds were elucidated as methyl 2,4,6- trihydroxy-3-(3-methylbut-2-enyl)benzoate (1), garcinisidone-A (2) and methyl 4,6dihydroxy-2-(4-methoxy-5- (3-methylbut-2-enyl)-3,6-dioxocylohexa-1,4-dienyloxy)-3-(3-methylbut-2-enyl)benzoate (3). Compound 1, 2 and 3 had IC50 value of 21.0 ± 10.2 μM, 21.2 ±8.4 μM and 17.2 ± 6.2μM against MCF-7, while only compound (2) was found to be in active against H-460 with IC50 value of 18.1 ± 6.7 μM. Conclusion: The results indicate that G. cowa leaves could be important sources of natural cytotoxic compounds and only compound (2) had activity against H-460 cell lines.

CHOLERETIC PLANT REMEDIES OF THE TRADITIONAL MEDICINE AND PROSPECTS OF THE CREATION OF NEW PREPARATIONS ON THEIR BASE / Монголын эм зүй, эм судлал

25 species of plants from Transbaikalian flora possess choleretic activity in a varying degree depending on the doses used. Choleretic activity was revealed in multicomponent remedies and xanthone compounds from Gentianaceae family.Key words: bile, plant mono- and complex plant remedies, xanthone compounds.IntroductionMedicinal plants were used as choleretic remedies since ancient times. In particular these plants are widely used for the treatment of digestive organs diseases which have the tendency to chronization and require complex long-term pharmacotherapy.The objective of the present work was to estimate the choleretic activity of the plants used for the treatment of hepatobiliary diseases in the traditional medicine.There were studied the decoctions from the aerial part of Lomatogonium carinthiacum, Gentianopsis barbata, Gentiana macrophylla, Halenia corniculata (Gentianaceae), Trifolium lupinaster, Parnassia palustris (Saxifragaceae), Leptopyrum fumarioides, Odontites rubra (Scrophulariaceae), Artemisia scoparia, Crepis tectorum (Asteraceae), Arctostaphylos uva-ursi (Ericaceae), Pyrola rotundifolia (Pyrolaceae), flowers of Vincetoxicum sibiricum (Asclepiadaceae), Hypecoum erectum (Papaveraceae), Hemerocalis minor (Liliaceae), branches of Myricaria dahurica (Tamaricaceae), leaves of Vaccinium vitis-idaea (Ericaceae), and fruits of Crataegus sanginea (Rosaceae).Besides there were studied the extracts from Trifolium repens (Fabaceae), Phlomis tuberosa (Lamiaceae), Pentaphylloides fruticosa, Polygonum aviculare (polygonaceae), Pedicularis verticillata, Linaria vulgaris (Scrophylariaceae) and Bergenia crassifolia (Saxifragaceae).Materials and methodsThe experiments were carried out on white male and female Wistar rats. The bile was obtained according to the generally accepted method [1] with the use of polyethylene cannula inserted into the common bile duct of the rats under barbamyle narcosis (0.8 ml per 100 g of weight, intraperitoneally). The bile was collected every hour for 5 hours. The power of choleretic activity of the obtained extract was estimated according to the secretion rate and total amount of the bile secreted as well as according to the content of the main ingredients in the bile, namely bilirubin [2], bile acids and cholesterol [3]. The decoctions were studied at the doses from 10 to 1000 mg/kg of the animal weight (on air-dry basis). The rats of the control group received the same volume of purified water. The data processing was performed with the use of the Student’s t-criterion.

Chemical Constituents from Hypericum beanii / 中草药·英文版

Objective: To study the chemical constituents from the aerial parts of Hypericum beanii. Methods: Various chromatographic techniques were used to separate the constituents and their structures were elucidated on the basis of extensive spectroscopic interpretation. Results: Fifteen compounds were isolated from the aerial parts of H. beanii. Their structures were identified as hyperbeanol E (. 1), (E)-linalool-1-oic acid (. 2), (4S,5R)-5-(4'-methyl-3'-pentenyl)-4-hydroxy-5-methyl-dihydrofuran-2-one (. 3), benzoic acid (. 4), 4-(3-O-3')-3'-methylbutenyl-6-phenyl-pyran-2-one (. 5), 4-hydroxy-4a,7-dimethoxy-4,4a-dihydrodibenzo-p-dioxin-2(3H)-one (. 6), isoimperatorin (. 7), 2,3-dimethoxyxanthone (. 8), 3,4-dihydroxy-2-methoxyxanthone (. 9), osajaxanthone (. 10), nigrolineaxanthone F (. 11), hypercohone G (. 12), betulinic acid (. 13), oleanolic acid 3β-caffeate (. 14), and isoastilbin (. 15). Conclusion: Compound 1 is a new menthane monoterpene derivative which owns an extra lactone ring. Compounds 2-7 and 10-15 are isolated from genus Hypericum Linn. for the first time and the other compounds are first obtained from the plants in H. beanii.

Biotransformation and involved CYP450 isomers of bioactive xanthone HM-1 from Halenia elliptica, D. Don in rat liver microsomes / 中国药学杂志

OBJECTIVE: To study the biotransformation of bioactive xanthones 1-hydroxyl-2, 3, 5-trimethoxyxanthone (HM-1) from Halenia elliptica, D. Don and five mostly common CYP450 isoforms involved of the its metabolism in rat liver microsomes (RLMs).

Fingerprint analysis of Zhimu-Huangbai herb pair and simultaneous determination of its alkaloids, xanthone glycosides and steroidal saponins by HPLC-DAD-ELSD / 中国天然药物

AIM@#To develop and validate a high performance liquid chromatography (HPLC) coupled with diode array and evaporative light scattering detectors (DAD-ELSD) method for the quantitative determination and fingerprint analysis of ten active constituents in three chemical classes (namely, xanthone glycosides, steroidal saponins, and alkaloids) in Zhimu-Huangbai herb pair (ZB).@*METHOD@#Chromatographic separation was performed on a Diamonsil C18 column (4.6 mm × 250 mm, 5 μm, Dikma) by gradient elution using acetic acid in acetonitrile solution at a flow rate of 1.0 mL·min(-1) at 260 nm. The drift tube temperature of ELSD was set to 60 °C and nebulizer gas pressure was 4.0 Bar. Method validation was performed to assure its linearity, limits of detection and quantification, precision, repeatability, stability, and accuracy.@*RESULTS@#The HPLC-DAD-ELSD method allowed the quantification of ten compounds (phellodendrine, jatrorrhizine, palmatine, berberine, neomangiferin, mangiferin, timosaponin E-I, timosaponin B-II, timosaponin B, and timosaponin A-III), and was successfully applied to fingerprint analysis for ten batches of ZB samples.@*CONCLUSION@#This was the first time to apply the combination of DAD and ELSD for the simultaneous determination of ten active ingredients in ZB. The results showed that the combination of quantitative analysis for marker ingredients and chemical fingerprint for the TCM herb pair provides a potentially powerful, widely introduced, and internationally accepted strategy for assessment of complex TCM formulas.

A New Prenylated Xanthone from Root Barks of Cudrania cochinchinensis / 中草药·英文版

Objective: To study the chemical constituents from the root barks of Cudrania cochinchinensis. Methods: The chemical constituents were isolated and purified by silica gel column chromatography. The structures of the compounds were identified on the basis of spectral data (MS, 1H-NMR, 13C-NMR, and 2D NMR) and by the comparison of spectroscopic data with the reported values in the literatures. Results: A new xanthone, 1,6,7-trihydroxy-4-(1,1-dimethylallyl)-3-methoxyxanthone (1) and a known prenylated xanthone 1,5,6-trihydroxy-4-(1,1-dimethylallyl)-3-methoxyxanthone (isocudraniaxanthone B, 2) were isolated from the root barks of C. cochinchinensis. Conclusion: Compound 1 is a new prenylated xanthone. Isomers 1 and 2 are obtained from this plant for the first time. © 2013 Tianjin Press of Chinese Herbal Medicines.